LysoView™ dyes are useful for staining lysosomes in live cells.
- No-wash, fluorescent staining of lysosomes in live cells
- Non-toxic for real-time live-cell imaging
- Lower background than LysoTracker® dyes
- Dye options for super-resolution imaging
- Color choices from blue to far-red, plus a unique NIR dye
LysoView™ dyes are fluorescent stains for imaging lysosome localization and morphology in live cells. The dyes accumulate in low pH organelles, resulting in highly specific staining without the need for a wash step. LysoView™ staining is non-toxic, and can imaged over the course of days, even after dye washout for some dye options (see the Product Information Sheet for more information).
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LysoView™ 540 and LysoView™ 633 exhibit pH-sensitive fluorescence, further enhancing the specificity of staining. LysoView™ 488 has been validated in super-resolution imaging by SIM (Ref. 7), while LysoView™ 650 fluorophore is compatible with super-resolution imaging by SIM and STED.
LysoView™ 540 and LysoView™ 633 stain vacuoles in yeast. Staining of bacteria varies between dyes. See our Cellular Stains Table for details.
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Note: LysoView™ 540 has limited photostability, and may not be suitable for all microscopy applications. LysoView™ 550 is a bright and more photostable choice.
Also see our unique UV-activatable Light-On LysoView™ 555, which is non-fluorescent until it is reversibly activated by UV light.
LysoView™ Dyes
Product Catalog number Size Abs/Em (nm) (pH ≤ 5) Detection channel Features LysoView™ 405, 1000X in DMSO 70066-T 10 uL 318, 400/ 464 DAPI, Pacific Blue™ Blue fluorescent lysosome stain 70066 50 uL LysoView™ 488, 1000X in DMSO 70067-T 10 uL 496/526 GFP, FITC Green fluorescent dye validated in SIM 70067 50 uL LysoView™ 540, 1000X in DMSO 70061-T 10 uL 540/634 TRITC, Cy®3, PE Orange, pH- sensitive fluorescence** 70061 50 uL LysoView™ 550, 1000X in DMSO 70083-T 10 uL 542/567 TRITC, Cy®3, PE Bright & photostable orange dye 70083 50 uL LysoView™ 594, 1000X in DMSO 70084-T 10 uL 585/634 Texas Red® Bright & photostable red dye 70084 50 uL LysoView™ 633 (lyophilized solid) 70058-T 1 vial* 634/657 Cy®5, APC Far-red, pH-sensitive fluorescence 70058 10 vials* LysoView™ 640, 1000X in DMSO 70085-T 10 uL 640/671 Cy®5, APC Bright & photostable far-red dye 70085 50 uL LysoView™ 650, 1000X in DMSO 70059-T 10 uL 650/675 Cy®5, APC Photostable far-red dye compatible with SIM, STED 70059 50 uL LysoView™ 680, 1000X in DMSO 70086-T 10 uL 674/711 Cy®5.5 Unique near-IR lysosome stain 70086 50 uL *Each vial of LysoView™ 633 makes 100 uL of 1000X dye after reconstitution. ** LysoView™ 540 has limited photostability and may not be suitable for all microcopy applications. Cy Dye is a registered trademark of Cytiva; Pacific Blue is a trademark and Texas Red is a registered trademark of Thermo Fisher Scientific.
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